Composite

Part:BBa_K2057004:Design

Designed by: José Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


LasR under the regulation of the promoter BtI-BtII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 715
    Illegal AgeI site found at 912
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As promoter is not available in the biobricks, we amplified, sequenced and characterized using the cry1Ac gene as template.


Source

Promoter BtI-BtII is harbor in a megaplasmid of B. thuringiensis, lasR was obtained from a biobrick.

References

(i) VON TERSCH MA, Robbins HL, Jany CS, Johnson TB (1991) Insecticidal toxins from Bacillus thuringiensis subsp. kenyae: gene cloning and characterization and comparison with B. thuringiensis subsp. kurstaki CryIA(c) toxins Appl. Environ. Microbiol. 57: 349-358.2. (II) SEDLAK M, Walter T, Aronson A (2000) Regulation by overlapping promoters of the rate of synthesis and deposition into crystalline inclusions of Bacillus thuringiensis delta-endotoxins. J Bacteriol. 182(3):734-41.